Plant Physiol. Journal of Pharmacology and Experimental Therapeutics
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Plant Physiology 85:808-815 (1987)
© 1987 American Society of Plant Biologists

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Molecular Biology and Gene Regulation

Plastid Development in Pisum sativum Leaves during Greening 1

I. A Comparison of Plastid Polypeptide Composition and in Organello Translation Characteristics

Karl-Josef Dietz2 and Lawrence Bogorad

Department of Cellular and Developmental Biology, Harvard University, Cambridge, Massachusetts 02138

Changes in plastid polypeptide composition during greening of etiolated peas were investigated by two-dimensional gel electrophoresis. One hundred of the more than 250 polypeptides which could be detected upon silver staining were followed during plastid development. Thirty-nine polypeptides decreased in abundance on a per organelle basis. Twentythree of the 46 polypeptides which increased in abundance upon greening could be identified as proteins of the thylakoid membrane. The changes in proteins observed during greening of etiolated leaves corresponded largely to those observed during normal leaf expansion. The origin of some of the polypeptides was traced back by comparing the two-dimensional gels of plastid proteins with in organello translation products and with polypeptides which had been synthesized in vitro from poly(A+) mRNA preparations and posttranslationally imported by chloroplasts. Some polypeptides were specifically identified in two-dimensional gels by Western blot analysis.

2 Supported by a grant of the Deutsche Forschungsgemeinschaft (DI 346). Present address: Lehrstuhl Botanik I der Universitaet, Mittlerer Dallenbergweg 64, 8700 Wuerzburg, West Germany.

1 Supported in part by a research grant from the National Institute of General Medical Sciences. It was also supported in part by the Maria Moors Cabot Foundation for Botanical Research of Harvard University.






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