Plant Physiology 85:916-921 (1987)
© 1987 American Society of Plant Biologists
Metabolism and Enzymology
Conversion of Xanthoxin to Abscisic Acid by Cell-Free Preparations from Bean Leaves 1
Ram K. Sindhu and
Daniel C. Walton
Department of Biology, State University of New York, Syracuse, New York 13210,
College of Environmental Science and Forestry, Syracuse, New York 13210
Cell-free extracts from the leaves of Phaseolus vulgaris L. convert xanthoxin to abscisic acid. The enzyme activity in dialyzed or acetone-precipitated extracts shows a strong dependence on either NAD or NADP. The enzyme activity appears to be cytosolic with no significant activity observed in chloroplasts. The activity was observed in extracts from roots of Phaseolus vulgaris, and also in extracts prepared from the leaves of Pisum sativum L., Zea mays L., Cucurbita maxima Duchesne, and Vigna radiata L. Neither water stress nor cycloheximide appear to significantly affect the level of enzyme activity in leaves. No intermediates between xanthoxin and abscisic acid were detected.
1 Supported by National Science Foundation Grant PCM 8219122.
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