This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Matsuoka, M. Articles by Ohashi, Y. Search for Related Content PubMed PubMed Citation Articles by Matsuoka, M. Articles by Ohashi, Y. Agricola Articles by Matsuoka, M. Articles by Ohashi, Y.

Molecular Biology and Gene Regulation

Classification and Structural Comparison of Full-Length cDNAs for Pathogenesis-Related Proteins ¹

National Institute of Agrobiological Resources, Tsukuba Science City, Ibaraki 305, Japan, Forestry and Forest Products Research Institute, Tsukuba Science City, Ibaraki 305, Japan, Fruits Tree Research Station, Tsukuba Science City, Ibaraki 305, Japan, National Institute of Agro-Environmental Science, Tsukuba Science City, Ibaraki 305, Japan, Department of Biology, College of Arts and Sciences, The University of Tokyo, Komaba, Tokyo 153, Japan

Fourteen cDNA clones of pathogenesis-related (PR) proteins, PR1a and PR1b of tobacco were obtained and classified into six groups based on restriction enzyme maps. To assign the groups to different classes of PR1 proteins, all the clones were partially sequenced and compared with amino acid sequences of PR1a and PR1b. Two groups of these corresponded to PR1a and four to PR1b. The results indicate that there are at least two kinds of PR1a mRNAs and four kinds of PR1b mRNAs. In fact, one cDNA insert hybridized to at least six to seven DNA fragments in restriction enzyme fragments of Samsun NN genomic DNA, indicating that the PR1 protein genes exist as a multigene family in the tobacco genome. Two sequences of essentially full-length cDNAs for PR1a and PR1b were determined and compared. The coding sequences of two cDNAs share 93% homology and the deduced amino acid sequences of PR1a and PR1b precursors, which are synthesized as larger precursors containing signal peptides, are 91% homologous. The homology of mature PR1a and PR1b regions is higher than that of larger precursors, 94% in the nucleotide sequence and 93% in the amino acid sequence, whereas that of the signal peptide regions is 80 and 90%, respectively. The hydropathy patterns and the secondary structures predicted by Chou-Fasman rules are similar to tomato PR protein in the half-side of the C terminus, which suggests that the half-C terminus side is important for the function of PR1 proteins.

This article has been cited by other articles:

				R. Takabatake, E. Karita, S. Seo, I. Mitsuhara, K. Kuchitsu, and Y. Ohashi Pathogen-Induced Calmodulin Isoforms in Basal Resistance Against Bacterial and Fungal Pathogens in Tobacco Plant Cell Physiol., March 1, 2007; 48(3): 414 - 423. [Abstract] [Full Text] [PDF]

				K. Sasaki, S. Hiraga, H. Ito, S. Seo, H. Matsui, and Y. Ohashi A Wound-Inducible Tobacco Peroxidase Gene Expresses Preferentially in the Vascular System Plant Cell Physiol., January 1, 2002; 43(1): 108 - 117. [Abstract] [Full Text] [PDF]

				S. Seo, M. Okamoto, T. Iwai, M. Iwano, K. Fukui, A. Isogai, N. Nakajima, and Y. Ohashi Reduced Levels of Chloroplast FtsH Protein in Tobacco Mosaic Virus-Infected Tobacco Leaves Accelerate the Hypersensitive Reaction PLANT CELL, June 1, 2000; 12(6): 917 - 932. [Abstract] [Full Text]

				S. Seo, M. Okamoto, H. Seto, K. Ishizuka, H. Sano, and Y. Ohashi Tobacco MAP Kinase: A Possible Mediator in Wound Signal Transduction Pathways Science, December 22, 1995; 270(5244): 1988 - 1992. [Abstract] [PDF]