Plant Physiol.
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Plant Physiology 86:359-363 (1988)
© 1988 American Society of Plant Biologists

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Metabolism and Enzymology

Different Rates of Metabolism of Two Chloroacetanilide Herbicides in Pioneer 3320 Corn

Keith M. O'Connell, E. Jay Breaux1 and Robert T. Fraley

Monsanto Company, 700 Chesterfield Village Parkway, Chesterfield, Missouri 63198

The in vivo rates of uptake and detoxification of alachlor and metolachlor were determined using Pioneer corn 3320 seedlings. Equal amounts of the radiolabeled herbicides were applied to etiolated coleoptiles and, at various intervals after treatment, the unabsorbed radioactivity was removed and quantified. Analysis of 80% methanol extracts by reverse phase liquid chromatography showed no significant differences in the rate of uptake of metolachlor and alachlor. However, the rate of glutathione conjugation of alachlor in vivo was two- to threefold greater than the rate for metolachlor at 2 and 4 hours after herbicide application. Since the initial step in detoxification is conjugation of the chloroacetanilide to glutathione, the activities of the enzymes responsible for conjugation, the glutathione-S-transferases (GST) were also analyzed in vitro, using crude extracts and the purified GST enzymes. The specific activities of the extracts were consistent with the results in vivo. Using alachlor as a substrate, the specific activity for glutathione conjugation was almost threefold higher than that for metolachlor. Kinetic analysis of purified GST III indicates that the enzyme has a higher affinity for alachlor (Kmapp = 1.69 millimolar) than for metolachlor (Kmapp = 8.9 millimolar).

1 Present address: Rhone Poulenc, Agrochimie, Lyons, France.




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