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Metabolism and Enzymology

Developmental Regulation of Enzymes of Indole Alkaloid Biosynthesis in Catharanthus roseus¹

Plant Biotechnology Institute, National Research Council, Saskatoon, Saskatchewan, Canada, S7N 0W9

Developing seedlings of Catharanthus roseus were analyzed for appearance of tryptophan decarboxylase (TDC), strictosidine synthase (SS), N-methyltransferase (NMT) and O-acetyltransferase (DAT) enzyme activities. SS enzyme activity appeared early after germination and was present throughout most of the developmental study. TDC activity was highly regulated and peaked over a 48 hour period achieving a maximum by day of 5 of seedling development. Both TDC and SS were present in all tissues of the seedling. NMT and DAT enzyme activities were induced after TDC and SS had peaked and these activities could only be found in hypocotyls and cotyledons. TDC, SS, and NMT did not require light for induction whereas DAT enzyme activity was increased approximately 10-fold after light treatment of dark grown seedlings.

³ Present address: Department of Biology, Acadia University, Wolfville, Nova Scotia, Canada, BOP 1X0.

¹ National Research Council of Canada No. 27933.

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