Plant Physiol. Journal of Pharmacology and Experimental Therapeutics
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Plant Physiology 86:510-511 (1988)
© 1988 American Society of Plant Biologists

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Metabolism and Enzymology

Hypochlorite Disinfection Influences the GA3-Induced Synthesis of {alpha}-Amylase Isozymes by Isolated Barley Aleurone Layers 1

Forrest T. Tittle, J. Stephen Goudey and Mary S. Spencer

Department of Plant Science, University of Alberta, Edmonton, Alberta T6G 2P5 Canada

Aleurone layers isolated from half-seeds of Himalaya barley (Hordeum vulgare cv Himalaya) disinfected in hypochlorite solutions containing 1.0% available chlorine synthesized significantly less {alpha}-amylase in response to gibberellic acid than layers derived from half-seeds disinfected in 0.1% hypochlorite. This effect of hypochlorite involved neither a differential decrease in the synthesis of group A or B {alpha}-amylase isozymes nor a general decrease in {alpha}-amylase synthesis attributable to fewer viable aleurone cells in layers from half-seeds disinfected with 1% hypochlorite. Our results emphasize the need to evaluate the potential effects of routine disinfection procedures used in physiological and biochemical studies.


1 Financial assistance from the Natural Sciences and Engineering Research Council of Canada (grant A-1451 to M. S. S.) is gratefully acknowledged.







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