Plant Physiology 86:510-511 (1988)
© 1988 American Society of Plant Biologists
Metabolism and Enzymology
Hypochlorite Disinfection Influences the GA3-Induced Synthesis of -Amylase Isozymes by Isolated Barley Aleurone Layers 1
Forrest T. Tittle,
J. Stephen Goudey and
Mary S. Spencer
Department of Plant Science, University of Alberta, Edmonton, Alberta T6G 2P5 Canada
Aleurone layers isolated from half-seeds of Himalaya barley (Hordeum vulgare cv Himalaya) disinfected in hypochlorite solutions containing 1.0% available chlorine synthesized significantly less -amylase in response to gibberellic acid than layers derived from half-seeds disinfected in 0.1% hypochlorite. This effect of hypochlorite involved neither a differential decrease in the synthesis of group A or B -amylase isozymes nor a general decrease in -amylase synthesis attributable to fewer viable aleurone cells in layers from half-seeds disinfected with 1% hypochlorite. Our results emphasize the need to evaluate the potential effects of routine disinfection procedures used in physiological and biochemical studies.
1 Financial assistance from the Natural Sciences and Engineering Research Council of Canada (grant A-1451 to M. S. S.) is gratefully acknowledged.
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