Plant Physiology 86:607-614 (1988)
© 1988 American Society of Plant Biologists
Environmental and Stress Physiology
Intracellular Compartmentation of Ions in Salt Adapted Tobacco Cells 1
Marla L. Binzel,
F. Dana Hess,
Ray A. Bressan and
Paul M. Hasegawa
Center for Plant Environmental Stress Physiology, Department of Horticulture, Purdue University, W. Lafayette, Indiana 47907,
Zoecon Research Institute, Sandoz Crop Protection Corporation, Palo Alto, California 94304
Na+ and Cl– are the principal solutes utilized for osmotic adjustment in cells of Nicotiana tabacum L. var Wisconsin 38 (tobacco) adapted to NaCl, accumulating to levels of 472 and 386 millimolar, respectively, in cells adapted to 428 millimolar NaCl. X-ray microanalysis of unetched frozen-hydrated cells adapted to salt indicated that Na+ and Cl– were compartmentalized in the vacuole, at concentrations of 780 and 624 millimolar, respectively, while cytoplasmic concentrations of the ions were maintained at 96 millimolar. The morphometric differences which existed between unadapted and salt adapted cells, (cytoplasmic volume of 22 and 45% of the cell, respectively), facilitated containment of the excited volume of the x-ray signal in the cytoplasm of the adapted cells. Confirmation of ion compartmentation in salt adapted cells was obtained based on kinetic analyses of 22Na+ and 36Cl– efflux from cells in steady state. These data provide evidence that ion compartmentation is a component of salt adaptation of glycophyte cells.
1 Supported in part by National Science Foundation grant PCM-8400133, United States Department of Agriculture grant 85-CRCR-1-1670 and Binational Agricultural Research and Development Fund grant US-961-85. Journal Paper 11,311, Purdue University Agricultural Experiment Station.
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