This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Web of Science (9) Citing Articles via Google Scholar Google Scholar Articles by Smrcka, A. V. Articles by Jensen, R. G. Search for Related Content PubMed PubMed Citation Articles by Smrcka, A. V. Articles by Jensen, R. G. Agricola Articles by Smrcka, A. V. Articles by Jensen, R. G.

Metabolism and Enzymology

HPLC Separation and Indirect Ultraviolet Detection of Phosphorylated Sugars ¹

Department of Biochemistry, University of Arizona, Tucson, Arizona 85721

An HPLC method for the separation and analysis of phosphorylated sugars is presented. Ion-exchange chromatography coupled to indirect ultraviolet detection has produced good resolution and sensitivity. Fructose 6-P, glucose 6-P, ribose 5-P, 3-phosphoglyceric acid, ribulose 1,5-P₂, fructose 1,6-P₂, and sedoheptulose 1,7-P₂ can be separated at a sensitivity down to 10 nanomoles. The system resolves 2-carboxy-D-arabinitol 1,5-P₂ from 2-carboxy-D-ribitol 1,5-P₂. The natural inhibitor of ribulose bisphosphate carboxylase, 2-carboxy-D-arabinitol 1-P, has been separated from its 5-P isomer and most other phosphorylated compounds. This method is applied to identification of the products obtained upon ion-exchange purification of synthetic 2-carboxyarabinitol 1-P.