Plant Physiol. Drug Metab Dispos
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Plant Physiology 86:822-825 (1988)
© 1988 American Society of Plant Biologists

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in Web of Science
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Web of Science (121)
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Chen, K.-H.
Right arrow Articles by Cohen, J. D.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Chen, K.-H.
Right arrow Articles by Cohen, J. D.
Agricola
Right arrow Articles by Chen, K.-H.
Right arrow Articles by Cohen, J. D.
Development and Growth Regulation

A Rapid and Simple Procedure for Purification of Indole-3-Acetic Acid Prior to GC-SIM-MS Analysis 1

Kai-Hsien Chen2, Anita N. Miller3, Glenn W. Patterson and Jerry D. Cohen

Department of Botany, University of Maryland, College Park, Maryland 20742, Department of Horticulture, University of Maryland, College Park, Maryland 20742, Plant Hormone Laboratory, Beltsville Agricultural Research Center-West, Beltsville, Maryland 20705

A simple and rapid procedure for the purification of indole-3-acetic acid prior to gas chromatography-selected ion monitoring-mass spectrographic analysis was developed using an amino anion exchange minicolumn and a short high resolution C18 column. Since multiple samples can be prepared at one time, the procedure is more rapid and the sample preparation time is reduced to one-third that normally required. In addition, the final recovery was improved by 40 to 50% over that of a solvent partitioning procedure.

2 Present address: Department of Pharmacology and Molecular Science, Johns Hopkins University, Baltimore, MD 21205.

3 Present address: Horticulture Department, Oregon State University, Corvallis, OR 97331.

1 This work was carried out under cooperative agreement No. 56-32U4-7-001 (RSA-7-001-010) of the United States Department of Agriculture/Agricultural Research Service and the University of Maryland and, in addition, was supported by a United States Israel BARD grant U.S.-842-84 and by National Science Foundation Metabolic Biology grant DMB-8617171.




This article has been cited by other articles:


Home page
 Plant Cell PhysiolHome page
A. Skirpan, A. H. Culler, A. Gallavotti, D. Jackson, J. D. Cohen, and P. McSteen
BARREN INFLORESCENCE2 Interaction with ZmPIN1a Suggests a Role in Auxin Transport During Maize Inflorescence Development
Plant Cell Physiol., March 1, 2009; 50(3): 652 - 657.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
B. Ehlert, M. A. Schottler, G. Tischendorf, J. Ludwig-Muller, and R. Bock
The paramutated SULFUREA locus of tomato is involved in auxin biosynthesis
J. Exp. Bot., October 1, 2008; 59(13): 3635 - 3647.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
Z. Chen, J. L. Agnew, J. D. Cohen, P. He, L. Shan, J. Sheen, and B. N. Kunkel
Pseudomonas syringae type III effector AvrRpt2 alters Arabidopsis thaliana auxin physiology
PNAS, December 11, 2007; 104(50): 20131 - 20136.
[Abstract] [Full Text] [PDF]

Home page
 ANN BOT (LOND)Home page
M. FAMBRINI, E. BONSIGNORI, F. RAPPARINI, G. CIONINI, V. MICHELOTTI, D. BERTINI, R. BARALDI, and C. PUGLIESI
stem fasciated, a Recessive Mutation in Sunflower (Helianthus annuus), Alters Plant Morphology and Auxin Level
Ann. Bot., October 1, 2006; 98(4): 715 - 730.
[Abstract] [Full Text] [PDF]

Home page
 DevelopmentHome page
P. Nagpal, C. M. Ellis, H. Weber, S. E. Ploense, L. S. Barkawi, T. J. Guilfoyle, G. Hagen, J. M. Alonso, J. D. Cohen, E. E. Farmer, et al.
Auxin response factors ARF6 and ARF8 promote jasmonic acid production and flower maturation
Development, September 15, 2005; 132(18): 4107 - 4118.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
J. L. Celenza, J. A. Quiel, G. A. Smolen, H. Merrikh, A. R. Silvestro, J. Normanly, and J. Bender
The Arabidopsis ATR1 Myb Transcription Factor Controls Indolic Glucosinolate Homeostasis
Plant Physiology, January 1, 2005; 137(1): 253 - 262.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
J. J. Campanella, A. F. Olajide, V. Magnus, and J. Ludwig-Muller
A Novel Auxin Conjugate Hydrolase from Wheat with Substrate Specificity for Longer Side-Chain Auxin Amide Conjugates
Plant Physiology, August 1, 2004; 135(4): 2230 - 2240.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
W. A. Peer, A. Bandyopadhyay, J. J. Blakeslee, S. N. Makam, R. J. Chen, P. H. Masson, and A. S. Murphy
Variation in Expression and Protein Localization of the PIN Family of Auxin Efflux Facilitator Proteins in Flavonoid Mutants with Altered Auxin Transport in Arabidopsis thaliana
PLANT CELL, July 1, 2004; 16(7): 1898 - 1911.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
H. P. d. Santos, E. Purgatto, H. Mercier, and M. S. Buckeridge
The Control of Storage Xyloglucan Mobilization in Cotyledons of Hymenaea courbaril
Plant Physiology, May 1, 2004; 135(1): 287 - 299.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
C. P. Keller, R. Stahlberg, L. S. Barkawi, and J. D. Cohen
Long-Term Inhibition by Auxin of Leaf Blade Expansion in Bean and Arabidopsis
Plant Physiology, March 1, 2004; 134(3): 1217 - 1226.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
Y. Zhao, A. K. Hull, N. R. Gupta, K. A. Goss, J. Alonso, J. R. Ecker, J. Normanly, J. Chory, and J. L. Celenza
Trp-dependent auxin biosynthesis in Arabidopsis: involvement of cytochrome P450s CYP79B2 and CYP79B3
Genes & Dev., December 1, 2002; 16(23): 3100 - 3112.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
S. Basu, H. Sun, L. Brian, R. L. Quatrano, and G. K. Muday
Early Embryo Development in Fucus distichus Is Auxin Sensitive
Plant Physiology, September 1, 2002; 130(1): 292 - 302.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. Walz, S. Park, J. P. Slovin, J. Ludwig-Muller, Y. S. Momonoki, and J. D. Cohen
A gene encoding a protein modified by the phytohormone indoleacetic acid
PNAS, February 5, 2002; 99(3): 1718 - 1723.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
J. C. Long, W. Zhao, A. M. Rashotte, G. K. Muday, and S. C. Huber
Gravity-Stimulated Changes in Auxin and Invertase Gene Expression in Maize Pulvinal Cells
Plant Physiology, February 1, 2002; 128(2): 591 - 602.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
D. E. Brown, A. M. Rashotte, A. S. Murphy, J. Normanly, B. W. Tague, W. A. Peer, L. Taiz, and G. K. Muday
Flavonoids Act as Negative Regulators of Auxin Transport in Vivo in Arabidopsis
Plant Physiology, June 1, 2001; 126(2): 524 - 535.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
G. Lobello, M. Fambrini, R. Baraldi, B. Lercari, and C. Pugliesi
Hormonal influence on photocontrol of the protandry in the genus Helianthus
J. Exp. Bot., August 1, 2000; 51(349): 1403 - 1412.
[Abstract] [Full Text] [PDF]

Home page
 Plant Physiol.Home page
Y. Y. Tam, E. Epstein, and J. Normanly
Characterization of Auxin Conjugates in Arabidopsis. Low Steady-State Levels of Indole-3-Acetyl-Aspartate, Indole-3-Acetyl-Glutamate, and Indole-3-Acetyl-Glucose
Plant Physiology, June 1, 2000; 123(2): 589 - 596.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
S. Grsic-Rausch, P. Kobelt, J. M. Siemens, M. Bischoff, and J. Ludwig-Müller
Expression and Localization of Nitrilase during Symptom Development of the Clubroot Disease in Arabidopsis
Plant Physiology, February 1, 2000; 122(2): 369 - 378.
[Abstract] [Full Text]

Home page
 Am. J. Bot.Home page
A. Ester Sztein, J. D. Cohen, I. G. de la Fuente, and T. J. Cooke
Auxin metabolism in mosses and liverworts
Am. J. Botany, November 1, 1999; 86(11): 1544 - 1555.
[Abstract] [Full Text] [PDF]

Home page
 Plant CellHome page
F. M. Carland, B. L. Berg, J. N. FitzGerald, S. Jinamornphongs, T. Nelson, and B. Keith
Genetic Regulation of Vascular Tissue Patterning in Arabidopsis
PLANT CELL, November 1, 1999; 11(11): 2123 - 2138.
[Abstract] [Full Text]

Home page
 Plant Physiol.Home page
R. C. Reed, S. R. Brady, and G. K. Muday
Inhibition of Auxin Movement from the Shoot into the Root Inhibits Lateral Root Development in Arabidopsis
Plant Physiology, December 1, 1998; 118(4): 1369 - 1378.
[Abstract] [Full Text]

Home page
 DevelopmentHome page
F. Carland and N. McHale
LOP1: a gene involved in auxin transport and vascular patterning in Arabidopsis
Development, January 6, 1996; 122(6): 1811 - 1819.
[Abstract] [PDF]

Home page
 ScienceHome page
A. D. WRIGHT, M. B. SAMPSON, M. G. NEUFFER, L. MICHALCZUK, J. P. SLOVIN, and J. D. COHEN
Indole-3-Acetic Acid Biosynthesis in the Mutant Maize orange pericarp, a Tryptophan Auxotroph
Science, November 15, 1991; 254(5034): 998 - 1000.
[Abstract] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY® THE PLANT CELL
Copyright © 1988 by the American Society of Plant Biologists