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Metabolism and Enzymology

Dual Role of Methionine in the Biosynthesis of Diacylglyceryltrimethylhomoserine in Chlamydomonas reinhardtii¹

Department of Botany, Faculty of Science, University of Tokyo, Hongo, Bunkyo-ku, Tokyo 113 Japan

Biosynthesis of the polar group of diacylglyceryl-O-4'-(N,N,N-trimethyl)homoserine (DGTS) was studied in intact cells of Chlamydomonas reinhardtii Dangeard. Among the three C₄ amino acids tested, only L-methionine could specifically inhibit the photosynthetic incorporation of [¹⁴C]NaHCO₃ into the polar group of DGTS. The radioactivity in L-[¹⁴C]methionine, which was labeled at either the C3 + C4, the C1, or the methyl carbon, was efficiently incorporated into the polar group of DGTS. These results suggest that the C₄ backbone and the S-methyl group of L-methionine are precursors to the C₄ backbone and the N-methyl groups of DGTS, respectively.

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