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Plant Physiology 86:1005-1007 (1988)
© 1988 American Society of Plant Biologists

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Environmental and Stress Physiology

Alterations in Membrane Protein-Profile during Cold Treatment of Alfalfa 1

Shyam S. Mohapatra, Ronald J. Poole and Rajinder S. Dhindsa

Centre for Plant Molecular Biology, McGill University, Montreal, PQ, Canada H3A 1B1, Department of Biology, McGill University, Montreal, PQ, Canada H3A 1B1

Changes in pattern of membrane proteins during cold acclimation of alfalfa have been examined. Cold acclimation for 2 to 3 days increases membrane protein content. Labeling of membrane proteins in vivo with [35S]methionine indicates increases in the rate of incorporation as acclimation progresses. Cold acclimation induces the synthesis of about 10 new polypeptides as shown by SDS-PAGE and fluorography of membrane proteins labeled in vivo.


1 Supported by F.C.A.C. (Quebec) grants AS-2241 and EQ-3146 to R. S. D. and R. J. P. and by Natural Sciences and Engineering Research Council of Canada, Canada grant A2724 to R. S. D.




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A. Bertrand, D. Prevost, F. J. Bigras, and Y. Castonguay
Elevated Atmospheric CO2 and Strain of Rhizobium Alter Freezing Tolerance and Cold-induced Molecular Changes in Alfalfa (Medicago sativa)
Ann. Bot., February 1, 2007; 99(2): 275 - 284.
[Abstract] [Full Text] [PDF]




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