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Plant Physiology 86:1127-1130 (1988)
© 1988 American Society of Plant Biologists

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Metabolism and Enzymology

31P-NMR Spectroscopy of Roots of Intact Corn Seedlings 1

Justin K. M. Roberts and M. Pia Testa

Department of Biochemistry, University of California, Riverside, California 92521

The application of 31P nuclear magnetic resonance spectroscopy to the study of metabolism in roots of intact corn seedlings is described. 31P-NMR spectra of developmentally distinct parts of primary roots of whole seedlings are presented. The spectra are of quality comparable to those of excised pieces of plant tissue.

1 Supported in part by a grant from the National Science Foundation (DMB 8521564). The GN500 NMR spectrometer was purchased in part from Department of Energy grant FG03-8613535, National Science Foundation grant DMB 8604091, and National Institutes of Health grant PHS BRSG 2 507.




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W. W.P. Chang, L. Huang, M. Shen, C. Webster, A. L. Burlingame, and J. K.M. Roberts
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Copyright © 1988 by the American Society of Plant Biologists