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Development and Growth Regulation

Positive Selection for Male-Sterile Mutants of Arabidopsis Lacking Adenine Phosphoribosyl Transferase Activity ¹

MSU-DOE Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824

Three mutants of Arabidopsis thaliana deficient in adenine phosphoribosyl transferase activity were isolated by selecting for germination of seeds on a medium containing 0.1 millimolar 2,6-diaminopurine. In each of the mutants, diaminopurine resistance was due to a recessive nuclear mutation at a locus designated apt. The mutants grow more slowly than wild type, and are male sterile due to abortion of pollen development after the meiotic divisions of the pollen mother cells. The reliability and ease with which the mutants can be selected should afford novel opportunities to investigate purine metabolism, pollen development, and genetic problems which require the ability to select for loss-of-function mutations.

¹ Supported in part by grants from the National Science Foundation (PCM8351595), DuPont de Nemours & Co., and the United States Department of Energy (DE-AC02-76ER01338).

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