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Metabolism and Enzymology

Inositol-Containing Lipids in Suspension-Cultured Plant Cells

An Isotopic Study

Department of Soil, Water and Plant Nutrition, Royal Veterinary- and Agricultural University, Thorvaldsensvej 40, 1871 Copenhagen C., Denmark, Division of Horticulture and Processing, D.S.I.R., Private Bag, Auckland, New Zealand, School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, Great Britain, Institute of Animal Physiology, AFRC, Babraham, Cambridge CB2 4AT, Great Britain

Polar lipids were extracted from suspension-cultured tomato (Lycopersicon esculentum Mill.) cells and analyzed by thin layer chromatography. Four major inositol-containing compounds were found, and incorporation of [³²P]orthosphosphate, [2-³H]glycerol, and myo-[2-³H]inositol was studied. Results showed that phosphatidylinositol-monophosphate is the phospholipid in these cells displaying the most rapid incorporation of [³²P]orthophosphate. We suggest that the tracer is incorporated primarily into the phosphomonoester group. Two inositol-containing lipids showed chromatographic behavior similar to phosphatidylinositol-4,5-bisphosphate when using standard thin layer chromatography techniques. The labeling pattern of these compounds, however, reveals that it is unlikely that either of these is identical to phosphatidylinositol-4,5-bisphosphate. Should phosphatidylinositol-bisphosphate be present in suspension cultured plant cells, our data indicate chemical abundancies substantially lower than previously reported.

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