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Plant Physiology 87:255-257 (1988)
© 1988 American Society of Plant Biologists

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Metabolism and Enzymology

Immunocytolocalization of Ferredoxin-GOGAT in the Cells of Green Leaves and Cotyledons of Lycopersicon esculentum1

Jose R. Botella, Jean P. Verbelen and Victoriano Valpuesta

Departmento de Bioquímica, Facultad de Ciencias, Universidad de Málaga, 29071 Málaga, Spain, Department Biologie, Universiteit Antwerpen U.I.A., Universiteitsplein 1, 2610 Wilrijk, Belgium

Ferredoxin-glutamate synthase was localized in leaves and cotyledons of tomato (Lycopersicon esculentum, cv Hellfrucht früshstamm) seedlings by immunocytochemical methods. The present work established that the enzyme was not only a constituent of chloroplast stroma of the mesophyll cells, but also of the cells of xylem parenchyma and epidermis.


1 Supported by a grant from the Comision Asesora de Investigación Científica y Técnica (3295/83. C3).




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Copyright © 1988 by the American Society of Plant Biologists