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Metabolism and Enzymology

Nucleoside Diphosphatase and 5'-Nucleotidase Activities of Soybean Root Nodules and Other Tissues ¹

Interdisciplinary Plant Physiology and Biochemistry Group, 204 Curtis Hall, University of Missouri-Columbia, Columbia, Missouri 65211

Nucleoside diphosphatase and 5'-nucleotidase activities were both found to be very high in extracts of soybean (Glycine max L.) root nodules. Both activities increased early in soybean nodule development, prior to the rise in leghemoglobin, and both were found at equivalent levels in nitrogenfixing and nonfixing nodules. Based on a survey of other tissues, these activities were both highest in soybean nodules (1300 nanomoles per milligram protein per minute, nucleoside diphosphatase and 500 nanomoles per milligram protein per minute, 5'-nucleotidase), but they were not always associated with each other; in some tissues one was high and the other low. Neither activity correlated well with ureide production; both seem, rather, to be primarily involved in some other metabolic function. Both the nucleoside diphosphatase and 5'-nucleotidase of soybean nodules were soluble proteins, and neither appeared to be associated with plastids, mitochondria, or bacteroids.

¹ Supported by the Missouri Agricultural Experiment Station and by a grant from the United States Department of Agriculture, Science and Education Administration, Competitive Grants Office, Grant 85-CRCR-1-1638. This research is a contribution of the Missouri Agricultural Experiment Station Journal Series No. 10411.