Plant Physiology 87:300-302 (1988)
© 1988 American Society of Plant Biologists
Molecular Biology and Gene Regulation
Chromosomal Location of Genes Encoding Barley (1 3, 1 4)- -Glucan 4-Glucanohydrolases 1
Lin Loi,
Bhavna Ahluwalia and
Geoffrey B. Fincher
Department of Biochemistry, La Trobe University, Bundoora, Victoria, 3083, Australia
Preparations of DNA from wheat (Triticum aestivum, cv Chinese Spring), barley (Hordeum vulgare, cv Betzes) and six euplasmic wheat-barley addition lines were digested to completion with restriction endonucleases and the products probed by Southern blot analysis using a cDNA-encoding barley (1 3, 1 4)- -glucanase isoenzyme II. It is shown that one of the barley (1 3, 1 4)- -glucanase genes is located on chromosome 1.
1 Supported by grants from the Barley Research Council of Australia (to G. B. F.).
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