Plant Physiol. Drug Metab Dispos
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Plant Physiology 87:427-430 (1988)
© 1988 American Society of Plant Biologists

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Metabolism and Enzymology

Nonaqueous Purification of Maize Mesophyll Chloroplasts 1

Hideaki Usuda

Laboratory of Chemistry, Faculty of Medicine, Teikyo University, Ohtsuka, Hachioji, Tokyo, Japan, 192-03

A nonaqueous fractionation method to obtain highly purified mesophyll chloroplasts from lyophilized leaves of Zea mays L. is described. The levels of several metabolites including pyruvate were determined in the purified mesophyll chloroplast fractions which were prepared from leaves exposed to different light intensities. The role of pyruvate in the regulation of pyruvate,Pi dikinase in these chloroplasts under different light intensities is discussed.

1 Partially supported by a grant from the Japanese Ministry of Education, Science, and Culture.






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Copyright © 1988 by the American Society of Plant Biologists