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Plant Physiology 87:671-674 (1988)
© 1988 American Society of Plant Biologists

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Molecular Biology and Gene Regulation

Stable Transformation of Soybean Callus by DNA-Coated Gold Particles

Paul Christou, Dennis E. McCabe and William F. Swain

Agracetus, Middleton, Wisconsin 53562

Immature soybean (Glycine max L.) embryos from commercially important cultivars were the targets of rapidly accelerated, DNA-coated, gold particles. Protoplasts were prepared from these tissues and propagated in culture under selection conditions for the introduced neomycin phosphotransferase II gene. Kanamycin-resistant calli were obtained at a rate of approximately 10–5. Enzyme assays and Southern blot hybridization confirmed the expression of the foreign gene and its stable integration into the soybean genome. Our results show that particle acceleration can be used for the introduction of foreign DNA into the soybean genome and indicate the technique may be useful in the recovery of engineered plants by transformation of regenerable tissues.




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Copyright © 1988 by the American Society of Plant Biologists