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Plant Physiology 87:781-784 (1988)
© 1988 American Society of Plant Biologists

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Development and Growth Regulation

Endogenous Gibberellins in Elongating Shoots of Clones of Salix dasyclados and Salix viminalis1

Olavi Junttila2, Hiroshi Abe3 and Richard P. Pharis

Plant Physiology Research Group, Department of Biology, The University of Calgary, Calgary, Alberta T2N 1N4, Canada

Elongating shoots of rapidly growing clones of Salix viminalis L. (clone 683-4) and Salix dasyclados Wimm. (clone 908) harvested in early August were analyzed for endogenous gibberellins (GA). Distribution of GA-like activity, determined by Tan-ginbozu dwarf rice microdrop bioassay after reverse phase C18 high performance chromatography, was similar for both species. For S. dasyclados, combined gas chromatography-selected ion monotoring (GC-SIM) yielded identifications of GA1, GA8, GA19, GA20, and GA29. Identifications of GA4 and GA9 were also made using co-injections of known amounts of [17, 17-2H2]GAs. By bioassay, the main activity was GA19-like in both species. Gibberellin A1, GA19, and GA20 concentrations were approximated by GC-SIM using co-injections of known amounts of [17,17-2H2]GAs. Both bioassay and GC-SIM results indicated very high concentrations of GA19 and GA20 (about 6000 nanograms per kilogram fresh weight shoot tissue using GC-SIM: 800 ng using bioassay), compared to the concentration of GA1 (about 130 nanograms per kilogram fresh weight using either GC-SIM or bioassay).

2 Present address: Institute of Biology and Geology, University of Tromsø, Box 3085 Guleng, N-9001 Tromsø, Norway.

3 Present address: Tokyo University of Agriculture and Technology, Plant Protection Department, Faculty of Agriculture, Fuchu, Tokyo, 183, Japan.

1 Supported by a NATO Travel Grant (No. 1647) to R. P. P. and O. J., as well as by the Natural Sciences and Engineering Research Council of Canada (grant A-2585) to R. P. P.






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