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Plant Physiology 88:16-20 (1988)
© 1988 American Society of Plant Biologists

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Molecular Biology and Gene Regulation

DNA Methylation Occurred around Lowly Expressed Genes of Plastid DNA during Tomato Fruit Development 1

Jarunya Ngernprasirtsiri2, Hirokazu Kobayashi and Takashi Akazawa

School of Agriculture, Nagoya University, Chikusa, Nagoya 464-01, Japan, Radioisotope Center, Nagoya University, Chikusa, Nagoya 464-01, Japan

We have analyzed DNA methylation of plastid DNA from fully ripened red fruits, green mature fruits, and green leaves of tomato (Lycopersicon esculentum var. Firstmore). Essentially identical restriction profiles were obtained between chromoplast and chloroplast DNAs by EcoRI digestion. BstNI/EcoRII and HpaII/MspI are pairs of isoschizomers that can discriminate between methylated and unmethylated DNAs. These endonucleases produced different restriction patterns of plastid DNAs from tomato fruits compared to tomato leaves. Moreover, we have found from Southern blots that methylation was not detected in DNA fragments containing certain genes that are actively expressed in chromoplasts, whereas DNA fragments bearing genes that are barely transcribed in chromoplasts are methylated.

2 Recipient of the predoctoral student fellowship provided by the Hitachi Scholarship Foundation (Tokyo).

1 This is paper No. 72 in the series "Structure of Chloroplast Proteins." The research was financially aided by a grant from the Ministry of Education, Science and Culture of Japan (Mombusho).






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