This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Web of Science (47) Citing Articles via Google Scholar Google Scholar Articles by Sindhu, R. K. Articles by Walton, D. C. Search for Related Content PubMed PubMed Citation Articles by Sindhu, R. K. Articles by Walton, D. C. Agricola Articles by Sindhu, R. K. Articles by Walton, D. C.

Environmental and Stress Physiology

Xanthoxin Metabolism in Cell-free Preparations from Wild Type and Wilty Mutants of Tomato ¹

Department of Biology, SUNY College of Environmental Science and Forestry, Syracuse, New York 13210

Extracts prepared from the turgid and water-stressed leaves of wild-type tomato (Lycopersicon esculentum Mill cv Ailsa Craig) and the wilty mutants sitiens, notabilis, and flacca were tested for their ability to metabolize xanthoxin to ABA. Extracts from wild type and notabilis converted xanthoxin at similar rates, while extracts from sitiens and flacca showed little or no activity. We also observed no activity when extracts of sitiens and flacca were mixed. Similar results were obtained when ABA aldehyde was used as a substrate, in that extracts from wild type and notabilis were equally active, but extracts from flacca and sitiens showed little activity. None of the tomato extracts showed significant activity with xanthoxin acid, xanthoxin alcohol, or ABA-1',4-'Trans-diol as substrates. Extracts from bean leaves (Phaseolus vulgaris L. cv Blue Lake) were similar to the wild-type tomato extracts in their ability to convert the various substrates to ABA, although excised bean leaves did convert ABA-1',4'-trans-diol and xanthoxin alcohol to ABA when these substances were taken up through the petiole. These results are consistent with a role for xanthoxin as a normal intermediate on the ABA biosynthetic pathway, and they suggest that ABA aldehyde is the final ABA precursor.

This article has been cited by other articles:

				M. Gonzalez-Guzman, N. Apostolova, J. M. Belles, J. M. Barrero, P. Piqueras, M. R. Ponce, J. L. Micol, R. Serrano, and P. L. Rodriguez The Short-Chain Alcohol Dehydrogenase ABA2 Catalyzes the Conversion of Xanthoxin to Abscisic Aldehyde PLANT CELL, August 1, 2002; 14(8): 1833 - 1846. [Abstract] [Full Text] [PDF]

				B.V. Milborrow The pathway of biosynthesis of abscisic acid in vascular plants: a review of the present state of knowledge of ABA biosynthesis J. Exp. Bot., June 1, 2001; 52(359): 1145 - 1164. [Abstract] [Full Text] [PDF]

				H. Hansen and K. Grossmann Auxin-Induced Ethylene Triggers Abscisic Acid Biosynthesis and Growth Inhibition Plant Physiology, November 1, 2000; 124(3): 1437 - 1448. [Abstract] [Full Text]

				J. T. Chernys and J. A.D. Zeevaart Characterization of the 9-Cis-Epoxycarotenoid Dioxygenase Gene Family and the Regulation of Abscisic Acid Biosynthesis in Avocado Plant Physiology, September 1, 2000; 124(1): 343 - 354. [Abstract] [Full Text]