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Plant Physiology 88:266-269 (1988)
© 1988 American Society of Plant Biologists

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Metabolism and Enzymology

High Performance Liquid Chromatography-Based Reevaluation of Disaccharides Produced upon Incubation of Sugarcane Vacuoles with UDP-Glucose 1

Andrew Maretzki and Margaret Thom

Hawaiian Sugar Planters' Association, Aiea, Hawaii 96701

A reanalysis of products formed after short-term incubation of sugarcane (Saccharum spp. hybrid cv H50-7209) vacuole preparations with uridine diphosphate [14C]glucose was performed. The results indicated that the ethanol-soluble substance previously identified as sucrose did not elute with sucrose when subjected to high performance liquid chromatography but had the same retention time as a disaccharide tentatively identified as laminaribiose.

1 Supported by a National Science Foundation grant (DMB 8613976). Published as Paper No. 649 in the journal series of the Experiment Station, Hawaiian Sugar Planters' Association.






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