This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Web of Science (38) Citing Articles via Google Scholar Google Scholar Articles by Sagar, A. D. Articles by Briggs, W. R. Search for Related Content PubMed PubMed Citation Articles by Sagar, A. D. Articles by Briggs, W. R. Agricola Articles by Sagar, A. D. Articles by Briggs, W. R.

Molecular Biology and Gene Regulation

Light Effects on Several Chloroplast Components in Norflurazon-Treated Pea Seedlings ¹

Carnegie Institution of Washington, Stanford, California 94305, Department of Plant Biology, Stanford, California 94305

Changes occurring in several chloroplast components during Norflurazon-induced photobleaching of Pisum sativum seedlings were investigated. mRNA steady state levels of the chlorophyll a/b-binding protein of photosystem II, ferredoxin I, the small and large subunits of ribulose 1,5-bisphosphate carboxylase, and pEA214 and pEA207, two other light-responsive genes, were determined during chlorophyll photooxidation. Relative transcription rates were assayed in isolated nuclei. The results illustrate a complex set of interactions regulating expression of the nuclear and chloroplast genomes. Photobleaching was found to affect the expression of the various genes in different ways. While transcript levels of the chlorophyll a/b-binding protein decreased by more than 80% under photooxidative light conditions in carotenoid-deficient peas, levels of ferredoxin, the small and large subunits of ribulose 1,5-bisphosphate carboxylase, and pEA214 mRNAs were reduced by less than 50%. pEA207 mRNA levels, on the other hand, were resistant to the effects of photobleaching. Analyses of chlorophylls a and b and the chlorophyll a/b-binding protein suggest that accumulation of the protein and its mRNA are coordinated with chlorophyll abundance at several steps. In addition to post-transcriptional regulation at the level of mRNA and protein stability, there may exist coordination at the transcriptional stage.

³ Present address: Department of Botany, North Carolina State University, Raleigh, NC 27695.

¹ This is Carnegie Institute of Washington publication No. 1010.

This article has been cited by other articles:

				S. M. Doyle, M. Diamond, and P. F. McCabe Chloroplast and reactive oxygen species involvement in apoptotic-like programmed cell death in Arabidopsis suspension cultures J. Exp. Bot., November 20, 2009; (2009) erp320v1. [Abstract] [Full Text] [PDF]

				Y. L. Chua, A. P. C. Brown, and J. C. Gray Targeted Histone Acetylation and Altered Nuclease Accessibility over Short Regions of the Pea Plastocyanin Gene PLANT CELL, March 1, 2001; 13(3): 599 - 612. [Abstract] [Full Text]