This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Web of Science (56) Citing Articles via Google Scholar Google Scholar Articles by Lincoln, J. E. Articles by Fischer, R. L. Search for Related Content PubMed PubMed Citation Articles by Lincoln, J. E. Articles by Fischer, R. L. Agricola Articles by Lincoln, J. E. Articles by Fischer, R. L.

Molecular Biology and Gene Regulation

Regulation of Gene Expression by Ethylene in Wild-Type and rin Tomato (Lycopersicon esculentum) Fruit ¹

Division of Molecular Plant Biology, University of California, Berkeley, California 94720

Levels of ethylene biosynthesis and ethylene-inducible gene expression in wild-type tomato (Lycopersicon esculentum) fruit and in nonripening fruit from the tomato mutant rin (ripening inhibitor) were compared in order to investigate the mechanism of ethylene action. Whereas wild-type tomato fruit dramatically increase the rate of ethylene biosynthesis at the onset of ripening, rin fruit constitutively produce ethylene at a low basal level. We have compared the mRNA levels and transcription rates of four cloned ethylene-inducible genes (JE Lincoln, S Cordes, E Read, RL Fischer 1987 Proc Natl Acad Sci USA 84: 2793-2797) during wild-type and rin fruit development. In wild-type fruit, both mRNA levels and transcription rates of these genes increase. The effect of the rin mutation on gene expression is different for each ethylene-inducible gene. In one case expression is completely suppressed, while in other instances it is either partially inhibited or relatively unaffected by the mutation. The mRNA levels of each of these genes in response to exogenous ethylene in rin fruit was also measured. The mRNAs for all four genes accumulate to similar levels in both ethylene treated rin and ethylene treated wild-type fruit. These results are discussed with regard to the response of plants to ethylene hormone at the level of gene expression.

This article has been cited by other articles:

				J. Vrebalov, I. L. Pan, A. J. M. Arroyo, R. McQuinn, M. Chung, M. Poole, J. Rose, G. Seymour, S. Grandillo, J. Giovannoni, et al. Fleshy Fruit Expansion and Ripening Are Regulated by the Tomato SHATTERPROOF Gene TAGL1 PLANT CELL, October 1, 2009; 21(10): 3041 - 3062. [Abstract] [Full Text] [PDF]

				D. Cantu, B. Blanco-Ulate, L. Yang, J. M. Labavitch, A. B. Bennett, and A. L.T. Powell Ripening-Regulated Susceptibility of Tomato Fruit to Botrytis cinerea Requires NOR But Not RIN or Ethylene Plant Physiology, July 1, 2009; 150(3): 1434 - 1449. [Abstract] [Full Text] [PDF]

				A. Tadiello, A. Pavanello, D. Zanin, E. Caporali, L. Colombo, G. L. Rotino, L. Trainotti, and G. Casadoro A PLENA-like gene of peach is involved in carpel formation and subsequent transformation into a fleshy fruit J. Exp. Bot., February 1, 2009; 60(2): 651 - 661. [Abstract] [Full Text] [PDF]

				F. Carrari and A. R. Fernie Metabolic regulation underlying tomato fruit development J. Exp. Bot., June 1, 2006; 57(9): 1883 - 1897. [Abstract] [Full Text] [PDF]

				R. Alba, P. Payton, Z. Fei, R. McQuinn, P. Debbie, G. B. Martin, S. D. Tanksley, and J. J. Giovannoni Transcriptome and Selected Metabolite Analyses Reveal Multiple Points of Ethylene Control during Tomato Fruit Development PLANT CELL, November 1, 2005; 17(11): 2954 - 2965. [Abstract] [Full Text] [PDF]

				C. S. Barry, R. P. McQuinn, A. J. Thompson, G. B. Seymour, D. Grierson, and J. J. Giovannoni Ethylene Insensitivity Conferred by the Green-ripe and Never-ripe 2 Ripening Mutants of Tomato Plant Physiology, May 1, 2005; 138(1): 267 - 275. [Abstract] [Full Text] [PDF]

				J. J. Giovannoni Genetic Regulation of Fruit Development and Ripening PLANT CELL, June 1, 2004; 16(suppl_1): S170 - S180. [Full Text] [PDF]

				L. Alexander and D. Grierson Ethylene biosynthesis and action in tomato: a model for climacteric fruit ripening J. Exp. Bot., October 1, 2002; 53(377): 2039 - 2055. [Abstract] [Full Text] [PDF]

				J. Vrebalov, D. Ruezinsky, V. Padmanabhan, R. White, D. Medrano, R. Drake, W. Schuch, and J. Giovannoni A MADS-Box Gene Necessary for Fruit Ripening at the Tomato Ripening-Inhibitor (Rin) Locus Science, April 12, 2002; 296(5566): 343 - 346. [Abstract] [Full Text] [PDF]

				C. S. Barry, M. I. Llop-Tous, and D. Grierson The Regulation of 1-Aminocyclopropane-1-Carboxylic Acid Synthase Gene Expression during the Transition from System-1 to System-2 Ethylene Synthesis in Tomato Plant Physiology, July 1, 2000; 123(3): 979 - 986. [Abstract] [Full Text]

				D. L. Smith and K. C. Gross A Family of at Least Seven beta -Galactosidase Genes Is Expressed during Tomato Fruit Development Plant Physiology, July 1, 2000; 123(3): 1173 - 1184. [Abstract] [Full Text]

				Y. Sitrit and A. B. Bennett Regulation of Tomato Fruit Polygalacturonase mRNA Accumulation by Ethylene: A Re-Examination Plant Physiology, March 1, 1998; 116(3): 1145 - 1150. [Abstract] [Full Text]