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Metabolism and Enzymology

Inorganic Carbon Accumulation by Chlamydomonas reinhardtii¹

New Proteins are made During Adaptation to Low CO₂

Department of Botany, Louisiana State University, Baton Rouge, Louisiana 70803

When the unicellular green alga Chlamydomonas reinhardtii is placed under low CO₂ conditions it adapts by making an inorganic carbon accumulating mechanism. Algal cells were labeled with ³⁵SO₄^–2 during this adaptation period and labeled proteins specific for this low CO₂ adaptation were identified. Four major proteins were preferentially synthesized under low CO₂ conditions and had M_r of 46, 44, 37, and 20 kilodaltons. The 37 kilodalton protein is most likely the periplasmic carbonic anhydrase previously identified as being part of the inorganic carbon accumulation mechanism of C. reinhardtii. The other three proteins have not been identified. The 46 and the 44 kilodalton proteins were not synthesized by a mutant algal strain, pmp-1, which cannot grow at low CO₂ concentrations. This strain does make the 37 and 20 kilodalton proteins, however. These data suggest that at least two or three proteins in addition to the periplasmic carbonic anhydrase are part of the inorganic carbon accumulation mechanism in C. reinhardtii.

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