Plant Physiol.
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Plant Physiology 88:943-946 (1988)
© 1988 American Society of Plant Biologists

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Metabolism and Enzymology

Immunocytolocalization of Glutamine Synthetase in Green Leaves and Cotyledons of Lycopersicon esculentum1

Jose R. Botella, Jean P. Verbelen and Victoriano Valpuesta

Departamento de Bioquímica, Facultad de Ciencias, Universidad de Málaga, 29071 Málaga, Spain, Departement Biologie, Universiteit Antwerpen U.I.A., Universiteitsplein 1, 2610 Wilrijk, Belgium

Glutamine synthetase was localized in leaves and cotyledons of young tomato (Lycopersicon esculentum Mill.) plants using immunogold techniques coupled to transmission electron microscopy. The enzyme occurs only in chloroplasts and is most probably a stroma constituent.

1 Supported by a Grant from the Dirección General de Investigación Científica y Técnica (PB86-0167-C03-03), and the Fonds voor Kallektief Fundamenteel Onderzoek (F.K.F.O.), Belgium.






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Copyright © 1988 by the American Society of Plant Biologists