Plant Physiol. email content delivery
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Plant Physiology 89:512-517 (1989)
© 1989 American Society of Plant Biologists

This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via CrossRef
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Slocum, R. D.
Right arrow Articles by Weinstein, L. H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Slocum, R. D.
Right arrow Articles by Weinstein, L. H.
Agricola
Right arrow Articles by Slocum, R. D.
Right arrow Articles by Weinstein, L. H.
Metabolism and Enzymology

Improved Method for HPLC Analysis of Polyamines, Agmatine and Aromatic Monoamines in Plant Tissue

Robert D. Slocum, Hector E. Flores, Arthur W. Galston and Leonard H. Weinstein

Department of Biology, Williams College, Williamstown, Massachusetts 01267, Biotechnology Institute, Pennsylvania State University, State College, Pennsylvania, Department of Biology, Yale University, New Haven, Connecticut 06520, Environmental Biology Program, Boyce Thompson Institute, Ithaca, New York 14853

The high performance liquid chromatographic (HPLC) method of Flores and Galston (1982 Plant Physiol 69: 701) for the separation and quantitation of benzoylated polyamines in plant tissues has been widely adopted by other workers. However, due to previously unrecognized problems associated with the derivatization of agmatine, this important intermediate in plant polyamine metabolism cannot be quantitated using this method. Also, two polyamines, putrescine and diaminopropane, also are not well resolved using this method. A simple modification of the original HPLC procedure greatly improves the separation and quantitation of these amines, and further allows the simulation analysis of phenethylamine and tyramine, which are major monoamine constituents of tobacco and other plant tissues. We have used this modified HPLC method to characterize amine titers in suspension cultured carrot (Daucus carota L.) cells and tobacco (Nicotiana tabacum L.) leaf tissues.




This article has been cited by other articles:


Home page
 Plant Physiol.Home page
R. Angelini, A. Tisi, G. Rea, M. M. Chen, M. Botta, R. Federico, and A. Cona
Involvement of Polyamine Oxidase in Wound Healing
Plant Physiology, January 1, 2008; 146(1): 162 - 177.
[Abstract] [Full Text] [PDF]

Home page
 J Exp BotHome page
L. Gemperlova, M. Novakova, R. Vankova, J. Eder, and M. Cvikrova
Diurnal changes in polyamine content, arginine and ornithine decarboxylase, and diamine oxidase in tobacco leaves
J. Exp. Bot., March 1, 2006; 57(6): 1413 - 1421.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
T. Kawano, R. Pinontoan, N. Uozumi, C. Miyake, K. Asada, P. E. Kolattukudy, and S. Muto
Aromatic Monoamine-Induced Immediate Oxidative Burst Leading to an Increase in Cytosolic Ca2+ Concentration in Tobacco Suspension Culture
Plant Cell Physiol., November 1, 2000; 41(11): 1251 - 1258.
[Abstract] [Full Text] [PDF]

Home page
 Plant Cell PhysiolHome page
T. Kawano, R. Pinontoan, N. Uozumi, Y. Morimitsu, C. Miyake, K. Asada, and S. Muto
Phenylethylamine-Induced Generation of Reactive Oxygen Species and Ascorbate Free Radicals in Tobacco Suspension Culture: Mechanism for Oxidative Burst Mediating Ca2+ Influx
Plant Cell Physiol., November 1, 2000; 41(11): 1259 - 1266.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
ASPB Publications PLANT PHYSIOLOGY THE PLANT CELL
Copyright © 1989 by the American Society of Plant Biologists