Plant Physiol. Journal of Pharmacology and Experimental Therapeutics
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Plant Physiology 89:525-529 (1989)
© 1989 American Society of Plant Biologists

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Metabolism and Enzymology

Detection of Norspermidine and Norspermine in Medicago sativa L. (Alfalfa) 1

Benjamin Rodriguez-Garay, Gregory C. Phillips and Glenn D. Kuehn

Department of Agronomy and Horticulture, Plant Genetic Engineering Laboratory, New Mexico State University, Las Cruces, New Mexico 88003, Department of Chemistry, Plant Genetic Engineering Laboratory, New Mexico State University, Las Cruces, New Mexico 88003

Shoot meristem tissues of alfalfa, Medicago sativa L., were found by high performance liquid chromatography analyses to contain the uncommon polyamines, norspermidine and norspermine. The chemical structures of norspermidine and norspermine, purified from alfalfa, were confirmed by comparison of mass spectra with those from authentic standards. The discovery of norspermidine and norspermine in alfalfa implicates the presence of at least two biosynthetic enzymes, a polyamine oxidase and a previously uncharacterized aminopropyltransferase.


1 Supported by a grant from the Southwest Consortium on Plant Genetics and Water Resources, 86-CRSR-2-2748. Journal article No. 1398 of the New Mexico Agricultural Experiment Station.




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P. Tavladoraki, M. N. Rossi, G. Saccuti, M. A. Perez-Amador, F. Polticelli, R. Angelini, and R. Federico
Heterologous Expression and Biochemical Characterization of a Polyamine Oxidase from Arabidopsis Involved in Polyamine Back Conversion
Plant Physiology, August 1, 2006; 141(4): 1519 - 1532.
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