This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Yoshida, S. Articles by Etani, S. Search for Related Content PubMed PubMed Citation Articles by Yoshida, S. Articles by Etani, S. Agricola Articles by Yoshida, S. Articles by Etani, S.

Environmental and Stress Physiology

Impairment of Tonoplast H⁺-ATPase as an Initial Physiological Response of Cells to Chilling in Mung Bean (Vigna radiata [L.] Wilczek) ¹

The Institute of Low Temperature Science, Hokkaido University, Sapporo, Japan 060

Biochemical alterations of cellular membranes in chilling-sensitive mung bean (Vigna radiata [L.] Wilczek) hypocotyls were investigated with reference to chilling injury. Reversible decreases in activities of tonoplast H⁺-ATPase and in vivo respiration became manifest within 24 hours of chilling when tissues suffered no permanent injury as assessed by electrolyte leakage and regrowth capacity. These changes were found to be the earliest cellular responses to chilling. A density-shift on a sucrose density gradient was observed in Golgi membranes early in the chilling treatment, suggesting that Golgi function and/or membrane biogenesis via the Golgi may have been altered upon chilling. After chilling more than 2 days, irreversible changes were generally produced in cellular membranes including the plasma membrane, endoplasmic reticulum, and mitochondria. Respiratory functions remained intact in mitochondria isolated from tissues prechilled for 24 hours, but were impaired after prechilling for 3 days. Given the important role of the tonoplast H⁺-ATPase in the active transport of ions and metabolites, the early decline in the tonoplast H⁺-ATPase activity may give rise to an alteration of the cytoplasmic environment and, consequently, trigger a series of degenerative reactions in the cells.

This article has been cited by other articles:

				K. Kasamo, M. Yamaguchi, and Y. Nakamura Mechanism of the Chilling-Induced Decrease in Proton Pumping across the Tonoplast of Rice Cells Plant Cell Physiol., July 1, 2000; 41(7): 840 - 849. [Abstract] [Full Text] [PDF]

				Y. Kawamura, K. Arakawa, M. Maeshima, and S. Yoshida Tissue Specificity of E Subunit Isoforms of Plant Vacuolar H+-ATPase and Existence of Isotype Enzymes J. Biol. Chem., February 25, 2000; 275(9): 6515 - 6522. [Abstract] [Full Text] [PDF]

				M. A. Gonzàlez-Meler, M. Ribas-Carbo, L. Giles, and J. N. Siedow The Effect of Growth and Measurement Temperature on the Activity of the Alternative Respiratory Pathway Plant Physiology, July 1, 1999; 120(3): 765 - 772. [Abstract] [Full Text]