Plant Physiol.
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Plant Physiology 89:768-775 (1989)
© 1989 American Society of Plant Biologists

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Development and Growth Regulation

Maturation of Soybean Somatic Embryos and the Transition to Plantlet Growth

Julie A. Buchheim1, Susan M. Colburn and Jerome P. Ranch2

United AgriSeeds, P.O. Box 4011, Champaign, Illinois 61820

The maturation of soybean (Glycine max L. Merr.) somatic embryos was characterized. Maturation was assayed by evaluating the ability of somatic embryos to make the transition to a plantlet through a germination-like process. Somatic embryos were organized from cotyledons of immature soybean embryos. Maturation of somatic embryos occurred on a Murashige-Skoog basal medium supplemented with activated charcoal and 0.28 molar sucrose. After 8 weeks on this medium, somatic embryos exhibited vigorous, high frequency development to plantlets. The "germination" frequency (conversion) of somatic embryos, and plantlet recovery frequency varied concurrently with maturation period. Conversion and plant recovery required no exogenous growth regulators. Desiccation of immature somatic embryos under controlled humidity regimes resulted in increased frequency of conversion of immature somatic embryos. Morphological abnormalities appeared in the somatic embryos, but few were detrimental to conversion velocity. There was little effect of genotype on conversion velocity or frequency.

1 Present address: Department of Entomology, 402 Life Sciences Bldg., Louisiana State University, Baton Rouge LA 70803.

2 Present address: American Cyanamid, P.O. Box 400, Princeton, NJ 08540.




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J. GROLL, D. J. MYCOCK, and V. M. GRAY
Effect of Medium Salt Concentration on Differentiation and Maturation of Somatic Embryos of Cassava (Manihot esculenta Crantz)
Ann. Bot., May 1, 2002; 89(5): 645 - 648.
[Abstract] [Full Text] [PDF]


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Copyright © 1989 by the American Society of Plant Biologists