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Metabolism and Enzymology

Phosphatidylinositol(4,5)bisphosphate and Phosphatidylinositol(4)phosphate in Plant Tissues ¹

Department of Biochemistry, Institute of Animal Physiology & Genetics Research, Babraham, Cambridge CB2 4AT, United Kingdom, School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, United Kingdom, Department of Molecular Cell Biology, University of Amsterdam, Kruislaan 318, Amsterdam 1098 SM, The Netherlands, John Innes Institute, Colney Lane, Norwich NR4 7WH, United Kingdom

Pea (Pisum sativum) leaf discs or swimming suspensions of Chlamydomonas eugametos were radiolabeled with [³H]myo-inositol or [³²P]Pi and the lipids were extracted, deacylated, and their glycerol moieties removed. The resulting inositol trisphosphate and bisphosphate fractions were examined by periodate degradation, reduction and dephosphorylation, or by incubation with human red cell membranes. Their likely structures were identified as D-myo-inositol(1,4,5)trisphosphate and D-myo-inositol(1,4,)-bisphosphate. It is concluded that plants contain phosphatidylinositol(4)phosphate and phosphatidylinositol(4,5)bisphosphate; no other polyphosphoinositides were detected.

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