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Metabolism and Enzymology

Isolation and Characterization of a Mutant of Chlamydomonas reinhardtii Deficient in the CO₂ Concentrating Mechanism ¹

Department of Botany, Louisiana State University, Baton Rouge, Louisiana 70803, Department of Chemistry, Lafayette College, Easton, Pennsylvania 18042, Department of Biochemistry, Michigan State University, East Lansing, Michigan 48824, Department of Biology, Indiana University, Bloomington, Indiana 47401

A Chlamydomonas reinhardtii mutant has been isolated that cannot grow photoautotrophically on low CO₂ concentrations but can grow on elevated CO₂. In a test cross, the high CO₂-requirement for growth showed a 2:2 segregation. This mutant, designated CIA-5, had a phenotype similar to previously identified mutants that were defective in some aspect of CO₂ accumulation. Unlike previously isolated mutants, CIA-5 did not have detectable levels of the periplasmic carbonic anhydrase, an inducible protein that participates in the acquisition of CO₂ by C. reinhardtii. CIA-5 also did not accumulate inorganic carbon to levels higher than could be accounted for by diffusion. This mutant strain did not synthesize any of the four polypeptides preferentially made by wild type C. reinhardtii when switched from an environment containing elevated CO₂ levels to an environment low in CO₂. It is concluded that this mutant fails to induce the CO₂ concentrating system and is incapable of adapting to low CO₂ conditions.

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