Plant Physiol. Illumina
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Plant Physiology 89:1039-1041 (1989)
© 1989 American Society of Plant Biologists

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Metabolism and Enzymology

Abscisic Acid Biosynthesis in Isolated Embryos of Zea mays L. 1

Douglas A. Gage2, Franklin Fong and Jan A. D. Zeevaart

Michigan State University-Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824, Department of Soil and Crop Sciences, Texas Agricultural Experiment Station, Texas A&M University, College Station, Texas 77843

Previous labeling experiments with 18O2 have supported the hypothesis that stress-induced abscisic acid (ABA) is synthesized through an indirect pathway involving an oxygenated carotenoid (xanthophyll) as a precursor. To investigate ABA formation under nonstress conditions, an 18O2 labeling experiment was conducted with isolated embryos from in vitro grown maize (Zea mays L.) kernels. Of the ABA produced during the incubation in 18O2, three-fourths contained a single 18O atom located in the carboxyl group. Approximately one-fourth of the ABA synthesized during the experiment contained two 18O atoms. These results suggest that ABA synthesized in maize embryos under nonstress conditions also proceeds via the indirect pathway, requiring a xanthophyll precursor. It was also found that the newly synthesized ABA was preferentially released into the surrounding medium.

2 Present address: Michigan State University-National Institutes of Health Mass Spectrometry Facility, Department of Biochemistry, Michigan State University, East Lansing, MI 48824.

1 Supported by the United States Department of Energy under contract DE-AC02-76ERO-1338 and the National Science Foundation through grants PCM 83-14321 (to J.A.D.Z.) and PCM 8402572 (to J. D. Smith, F. Fong, and C. W. Magill).






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