Plant Physiol. Drug Metab Dispos
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Plant Physiology 89:1207-1212 (1989)
© 1989 American Society of Plant Biologists

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Metabolism and Enzymology

Regulation of Pea Mitochondrial Pyruvate Dehydrogenase Complex 1

Does Photorespiratory Ammonium Influence Mitochondrial Carbon Metabolism?

Kathryn A. Schuller and Douglas D. Randall

Department of Biochemistry, University of Missouri, Columbia, Missouri 65211

Inactivation of the pyruvate dehydrogenase complex catalyzed by pyruvate dehydrogenase kinase was studied using intact mitochondria purified from green leaf tissue of pea (Pisum sativum L.) and dialyzed mitochondrial extracts. Thiamine pyrophosphate was inhibitory in dialyzed extracts but not in intact mitochondria, except in the presence of high concentrations of Na+. NH4+, at concentrations as low as 20 micromolar, markedly stimulated inactivation in dialyzed extracts. K+ in the range 1 to 10 millimolar also enhanced inactivation. In contrast, Na+ was without affect at lower concentrations but was inhibitory at 10 to 100 millimolar levels. The effect of NH4+ is discussed in relation to a possible regulatory interaction between photorespiratory NH4+ production and the entry of carbon into the tricarboxylic acid cycle by way of the pyruvate dehydrogenase complex.

1 Supported by National Science Foundation grant DMB-8506473 and by the Missouri Agricultural Experiment Station. K. A. S. is the recipient of a Food For 21st Century Postdoctoral Fellowship. This is journal report number 10694 from the Missouri Agricultural Experiment Station.




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Copyright © 1989 by the American Society of Plant Biologists