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Plant Physiology 89:1299-1305 (1989)
© 1989 American Society of Plant Biologists

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Metabolism and Enzymology

Characterization of a meta-Fluorotyrosine-Tolerant Cell Culture of Eschscholtzia californica Cham

Jochen Berlin1, Robert B. van Huystee2, Ludger Witte, Jürgen Bender, Hans J. Jäger and Victor Wray

GBF-Gesellschaft für Biotechnologische Forschung m.b.H., Braunschweig, Federal Republic of Germany, Department of Plant Sciences, University of Western Ontario, London, Canada, Institut für Pharmateutische Biologie der Technischen Universität, Braunschweig, Federal Republic of Germany, Institut für Produktions- und Ökotoxikologie der Bundesforschungsanstalt für Landwirtschaft, Braunschweig, Federal Republic of Germany

A cell line of Eschscholtzia californica selected for meta-fluorotyrosine (MFT) tolerance was found to have 10-fold increased levels of phenylalanine and tyrosine compared to the parent line, while most other amino acids were only increased 2-fold. Tracer experiments with shikimic acid in the presence of MFT showed that the biosynthesis of the aromatic amino acids was not impaired in the tolerant line. Feeding experiments with phenylalanine, tyrosine, or shikimic acid also revealed a reduced turnover of the pools of the aromatic amino acids in the variant. Thus undisturbed de novo biosynthesis of the aromatic amino acids and dilution of toxic effects of MFT by the enlarged pool sizes seemed to be the main reason for the acquired tolerance. Despite the enlarged availability of the precursor tyrosine, formation of the benzophenanthridine alkaloids was enhanced neither in the growth nor in the production medium.

1 Present address: Institu für Biochemie, Biologische Bundesanstalt, Messeweg 11/12, D-3300 Braunschweig, FRG.

2 On sabbatical leave at the GBF.






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Copyright © 1989 by the American Society of Plant Biologists