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Plant Physiology 90:21-24 (1989) © 1989 American Society of Plant Biologists Effect of 2,5-Norbornadiene upon Ethylene Biosynthesis in Midclimacteric Carnation FlowersNPI, 417 Wakara Way, Salt Lake City, Utah 84108 The climacteric increase in ethylene production in carnation (Dianthus caryophyllus L. cv White Sim) flowers is known to be accompanied by an increase in 1-aminocyclopropane-1-carboxylate (ACC) synthase and ethylene forming enzyme (EFE) activities. When midclimacteric flowers were exposed to 2,5-norbornadiene, which blocks ethylene action, ethylene production began to decrease after 2 to 3 hours. ACC synthase activity was markedly reduced after 4 hours and the increase in EFE activity was blocked indicating that the autocatalytic signal associated with ethylene action stimulates both enzyme activities.
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