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Plant Physiology 90:75-84 (1989)
© 1989 American Society of Plant Biologists

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Metabolism and Enzymology

Immunological Comparison of the Starch Branching Enzymes from Potato Tubers and Maize Kernels 1

Greetje H. Vos-Scheperkeuter, Janny G. de Wit, Anne S. Ponstein, Will J. Feenstra and Bernard Witholt

Groningen Biotechnology Center, Department of Biochemistry, Nijenborgh 16, 9747 AG Groningen, The Netherlands, Department of Genetics, Kerklaan 30, 9751 NN Haren, The Netherlands

Starch branching enzyme was purified from potato (Solanum tuberosum L.) tubers as a single species of 79 kilodaltons and specific antibodies were prepared against both the native enzyme and against the gel-purified, denatured enzyme. The activity of potato branching enzyme could only be neutralized by antinative potato branching enzyme, whereas both types of antibodies reacted with denatured potato branching enzyme. Starch branching enzymes were also isolated from maize (Zea mays L.) kernels. All of the denatured forms of the maize enzyme reacted with antidenatured potato branching enzyme, whereas recognition by antinative potato branching enzyme was limited to maize branching enzymes I and IIb. Antibodies directed against the denatured potato enzyme were unable to neutralize the activity of any of the maize branching enzymes. Antinative potato branching enzyme fully inhibited the activity of maize branching enzyme I; the neutralized maize enzyme was identified as a 82 kilodalton protein. It is concluded that potato branching enzyme (Mr = 79,000) shares a high degree of similarity with maize branching enzyme I (Mr = 82,000), in the native as well as the denatured form. Cross-reactivity between potato branching enzyme and the other forms of maize branching enzyme was observed only after denaturation, which suggests mutual sequence similarities between these species.

1 This research was financed by the Department of Economic Affairs, the Hague, the Netherlands, through the ISP program. Some of the results presented in this paper were reported at the 2nd International Workshop on Plant Polysaccharides held in July 1987 in Grenoble, France; the abstract was published in a special issue of Food Hydrocolloids (21).






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Copyright © 1989 by the American Society of Plant Biologists