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Molecular Biology and Gene Regulation

An Examination of the Plastid DNA of Hypohaploid Nicotiana plumbaginifolia Plants

Department of Chemistry, University of Southern Mississippi, Hattiesburg, Mississippi 39401, Institut De Physiologie Vegetale, C.N.R.S., F-91190 Gif-sur-Yvette, France, Department of Cell and Developmental Biology, Roche Institute of Molecular Biology, Nutley, New Jersey 07110

DNA was extracted from different morphological types of hypohaploid Nicotiana plumbaginifolia plants. The cellular levels of chloroplast DNA (expressed as percent of total DNA) were found to be approximately two- to threefold higher in two albino hypohaploids than in a green hypohaploid. The level of chloroplast DNA in the green hypohaploid was not significantly different from either in vitro or in vivo grown haploid N. plumbaginifolia plants. Molecular hybridization with DNA probes for the large subunit of ribulose bisphosphate carboxylase from spinach and with Pvull fragments representing the entire Nicotiana tabacum chloroplast genome revealed no gross qualitative differences in the chloroplast DNAs of hypohaploid plants. Based on these observations we have concluded that the lack of chloroplast function observed in the albino forms of hypohaploid N. plumbaginifolia plants is not due to changes in the chloroplast genome.