This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Smith, V. A. Articles by MacMillan, J. Search for Related Content PubMed PubMed Citation Articles by Smith, V. A. Articles by MacMillan, J. Agricola Articles by Smith, V. A. Articles by MacMillan, J.

Development and Growth Regulation

An Immunological Approach to Gibberellin Purification and Quantification ¹

School of Chemistry, University of Bristol, Bristol BS8 1TS, United Kingdom

Gibberellin (GA) specific, high-affinity monoclonal antibodies have been used to assay the GA content of various plant tissues and to purify selected GAs by immunoaffinity chromatography. These immunological techniques may not stand alone as a general method of GA analysis. The results of this study indicate, however, that in conjunction with gas chromatography-mass spectrometry for positive GA identification, radioimmunoassay and immunoaffinity chromatography are extremely powerful tools for purifying and quantifying GAs from plant tissues.