Plant Physiology 90:1233-1238 (1989)
© 1989 American Society of Plant Biologists
Environmental and Stress Physiology
Topography of Photosynthetic Activity of Leaves Obtained from Video Images of Chlorophyll Fluorescence 1
Paul F. Daley,
Klaus Raschke,
J. Timothy Ball and
Joseph A. Berry
University of California, Lawrence Livermore National Laboratory, Environmental Sciences Division, P. O. Box 808/L-528, Livermore, California 94550,
Pflanzenphysiologisches Institut der Universitat Göttingen, Untere Karspüle 2, 3400 Göttingen, West Germany,
Botany Department, University of California, Davis, California 95616,
Carnegie Institution, Department of Plant Biology, 290 Panama St., Stanford, California 94305
The distribution of photosynthetic activity over the area of a leaf and its change with time was determined (at low partial pressure of O2) by recording images of chlorophyll fluorescence during saturating light flashes. Simultaneously, the gas exchange was being measured. Reductions of local fluorescence intensity quantitatively displayed the extent of nonphotochemical quenching; quench coefficients, qN, were computed pixel by pixel. Because rates of photosynthetic electron transport are positively correlated with (1 – qN), computed images of (1 – qN) represented topographies of photosynthetic activity. Following application of abscisic acid to the heterobaric leaves of Xanthium strumarium L., clearly delineated regions varying in nonphotochemical quenching appeared that coincided with areoles formed by minor veins and indicated stomatal closure in groups.
1 Support for the Trapix system was provided by the University of California Program for Analytical Cytology. Work by P. F. D. was performed under U.S. Department of Energy contract W-7405-Eng.-48 to the Lawrence Livermore National Laboratory. J. T. B. held a postdoctoral fellowship at the Botany Department at U.C. Davis. K. R. held a fellowship from the Stiftung Volkswagenwerk. This is C.I.W. Publication 1047.
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