Plant Physiol. Illumina
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Plant Physiology 90:1246-1248 (1989)
© 1989 American Society of Plant Biologists

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Metabolism and Enzymology

Carbohydrates Stimulate Ethylene Production in Tobacco Leaf Discs 1

III. Stimulation of Enzymic Hydrolysis of Indole-3-Acetyl-L-Alanine

Shimon Meir, Joseph Riov, Sonia Philosoph-Hadas and Nehemia Aharoni

Department of Fruit and Vegetable Storage, Agricultural Research Organization, The Volcani Center, Bet Dagan 50250, Israel, Department of Horticulture, Faculty of Agriculture, The Hebrew University of Jerusalem, Rehovot 76100, Israel

The sucrose-stimulated in vivo hydrolysis of indole-3-acetyl-L-alanine (IAAIa) in tobacco (Nicotiana tabacum L.) leaf discs was confirmed by in vitro analysis of an IAAIa-hydrolyzing enzyme isolated from the same tissue. The enzymic activity could be stimulated by either aging of the tissue or by application of external IAA or sucrose. A combination of the above three treatments yielded maximal activity.

1 Supported by grant No. I-773-84 from BARD, the United States-Israel Binational Agricultural Research and Development Fund. Contribution from the Agricultural Research Organization, the Volcani Center, Bet Dagan, Israel. No. 2540-E, 1988 series. Parts I and II in this series: Plant Physiol (1985) 78: 131-143.






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Copyright © 1989 by the American Society of Plant Biologists