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Development and Growth Regulation

-D-Glucan Antibodies Inhibit Auxin-Induced Cell Elongation and Changes in the Cell Wall of Zea Coleoptile Segments ¹

Department of Biology, Faculty of Science, Osaka City University, Sumiyoshi-ku, Osaka 558, Japan, Department of Vegetable Crops, University of California, Davis, California 95616

Antiserum was raised against the Avena sativa L. caryopsis -D-glucan fraction with an average molecular weight of 1.5 x 10⁴. Polyclonal antibodies recovered from the serum after Protein A-Sepharose column chromatography precipitated when cross-reacted with high molecular weight (13), (14)--D-glucans. These antibodies were effective in suppression of cell wall autohydrolytic reactions and auxin-induced decreases in noncellulosic glucose content of the cell wall of maize (Zea mays L.) coleoptiles. The results indicate antibody-mediated interference with in situ -D-glucan degradation. The antibodies at a concentration of 200 micrograms per milliliter also suppress auxin-induced elongation by about 40% and cell wall loosening (measured by the minimum stress-relaxation time of the segments) of Zea coleoptiles. The suppression of elongation by antibodies was imposed without a lag period. Auxin-induced elongation, cell wall loosening, and chemical changes in the cell walls were near the levels of control tissues when segments were subjected to antibody preparation precipitated by a pretreatment with Avena caryopsis -D-glucans. These results support the idea that the degradation of (13), (14)--D-glucans by cell wall enzymes is associated with the cell wall loosening responsible for auxin-induced elongation.

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