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Plant Physiology 90:1422-1428 (1989)
© 1989 American Society of Plant Biologists

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Metabolism and Enzymology

Separation and Characterization of Inositol Phospholipids from the Pulvini of Samanea saman1

Gary G. Coté, Anthony L. DePass2, Lynne M. Quarmby, Bonnie F. Tate, M. J. Morse3, Ruth L. Satter and Richard C. Crain

Department of Molecular and Cell Biology U-125, University of Connecticut, Storrs, Connecticut 06269

To supplement current thin-layer chromatographic methods for separation and quantitation of plant phospholipids, an alternative method, high-performance liquid chromatography was developed. The major inositol-containing lipids from the pulvini of Samanea saman Merr. were identified as phosphatidylinositol, phosphatidylinositol phosphate, and phosphatidylinositol bisphosphate based on comigration with authentic standards on high-performance liquid chromatography and on thin-layer chromatography. The patterns of incorporation of radioactivity into the putative phosphatidylinositol and phosphatidylinositol phosphate were consistent with these identifications when pulvini were labeled with [3H]glycerol, [3H]inositol, or [32P]orthophosphate. Analysis of the products of enzymic hydrolysis, of chemical deacylation, and of `fingerprint' methanolysis of these phospholipids confirmed the identifications.

2 Present address: Program in Molecular and Cell Biology, University of Massachusetts, Amherst, MA 01003.

3 Present address: Department of Botany, Connecticut College, New London, CT 06320.

1 This work was supported by National Science Foundation (NSF) grant DMB 86-07857 to R. C. C. and R. L. S., and by NSF grants DMB 83-04613 and DCB 88-04810 to R. L. S.




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C. Pical, T. Westergren, S. K. Dove, C. Larsson, and M. Sommarin
Salinity and Hyperosmotic Stress Induce Rapid Increases in Phosphatidylinositol 4,5-Bisphosphate, Diacylglycerol Pyrophosphate, and Phosphatidylcholine in Arabidopsis thaliana Cells
J. Biol. Chem., December 31, 1999; 274(53): 38232 - 38240.
[Abstract] [Full Text] [PDF]


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Copyright © 1989 by the American Society of Plant Biologists