This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Web of Science (33) Citing Articles via Google Scholar Google Scholar Articles by Huang, C. X. Articles by Van Steveninck, R. F. M. Search for Related Content PubMed PubMed Citation Articles by Huang, C. X. Articles by Van Steveninck, R. F. M. Agricola Articles by Huang, C. X. Articles by Van Steveninck, R. F. M.

Environmental and Stress Physiology

Maintenance of Low Cl^– Concentrations in Mesophyll Cells of Leaf Blades of Barley Seedlings Exposed to Salt Stress

School of Agriculture, La Trobe University, Bundoora, Victoria, 3083, Australia

The concentrations of vacuolar Na⁺ and Cl^– in the epidermal and mesophyll cells of the leaf blade and sheath of Hordeum vulgare seedlings (cv California Mariout and Clipper) were measured by means of quantitative electron probe x-ray microanalysis. A preferential accumulation of Cl^– in vacuoles of epidermal cells in both blade and sheath and a low level in mesophyll cells of the blade were evident in plants grown in full strength Johnson solution. The concentration of Cl^– in the mesophyll cells of the blade remained at a low level after exposure to 50 or 100 millimolar NaCl for 1 day or to 50 millimolar for 4 days, while at the same time the concentration of Cl^– in the epidermis and mesophyll of the sheath showed a dramatic increase. Clipper generally contained more Cl^– in the mesophyll cells of the blade than California Mariout. A greater accumulation of Na⁺ in the mesophyll of the sheath relative to that of the blade was only apparent after treatment with 100 millimolar NaCl for 1 day or 50 millimolar for 4 days. These results confirm the suggestion that sheath tissue is capable of accumulating excess Cl^– (and to a lesser extent Na⁺) and suggest that the site of regulation of Cl^– concentration in the barley leaf is located in the mesophyll cells of the blade.

This article has been cited by other articles:

				M. W. Shane, M. E. McCully, and H. Lambers Tissue and cellular phosphorus storage during development of phosphorus toxicity in Hakea prostrata (Proteaceae) J. Exp. Bot., May 1, 2004; 55(399): 1033 - 1044. [Abstract] [Full Text] [PDF]

				M. R. GIBBERD, N. C. TURNER, and R. STOREY Influence of Saline Irrigation on Growth, Ion Accumulation and Partitioning, and Leaf Gas Exchange of Carrot (Daucus carota L.) Ann. Bot., December 1, 2002; 90(6): 715 - 724. [Abstract] [Full Text] [PDF]

				A. J. Karley, R. A. Leigh, and D. Sanders Differential Ion Accumulation and Ion Fluxes in the Mesophyll and Epidermis of Barley Plant Physiology, March 1, 2000; 122(3): 835 - 844. [Abstract] [Full Text] [PDF]