This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in Web of Science Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Web of Science (61) Citing Articles via Google Scholar Google Scholar Articles by Hurkman, W. J. Articles by Tanaka, C. K. Search for Related Content PubMed PubMed Citation Articles by Hurkman, W. J. Articles by Tanaka, C. K. Agricola Articles by Hurkman, W. J. Articles by Tanaka, C. K.

Environmental and Stress Physiology

A Comparison of the Effect of Salt on Polypeptides and Translatable mRNAs in Roots of a Salt-Tolerant and a Salt-Sensitive Cultivar of Barley

U.S. Department of Agriculture, Agricultural Research Service, Western Regional Research Center, Plant Development-Productivity, Research Units, Albany, California 94710, Plant Development-Quality, Research Units, Albany, California 94710

The effect of salt stress on polypeptide and mRNA levels in roots of two barley (Hordeum vulgare L.) cultivars differing in salt tolerance (cv CM 72, tolerant; cv Prato, sensitive) was analyzed using two-dimensional polyacrylamide gel electrophoresis. Preliminary experiments indicated that germination of Prato was inhibited significantly in the presence of NaCl, but growth of the surviving Prato seedlings was not substantially different from that of CM 72. Fluorographs of two-dimensional gels containing in vivo labeled polypeptides or in vitro translation products were computer analyzed to identify and quantitate changes that resulted when plants were grown in the presence of 200 millimolar NaCl for 6 days. The patterns of in vivo labeled polypeptides and in vitro products of CM 72 and Prato were qualitatively the same. Salt caused quantitative changes in numerous polypeptides and translatable mRNAs, but, overall, the changes were relatively small. Salt did not induce the synthesis of unique polypeptides or translatable mRNAs and did not cause any to disappear. Because of the similarities of the two cultivars with respect to growth and polypeptide patterns and the slight changes in polypeptide and translation product levels caused by salt, specific polypeptides or translatable mRNAs that are related to salt tolerance in barley could not be identified.

This article has been cited by other articles:

				F. Riccardi, P. Gazeau, D. de Vienne, and M. Zivy Protein Changes in Response to Progressive Water Deficit in Maize . Quantitative Variation and Polypeptide Identification Plant Physiology, August 1, 1998; 117(4): 1253 - 1263. [Abstract] [Full Text]