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Plant Physiology 91:190-192 (1989)
© 1989 American Society of Plant Biologists

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Environmental and Stress Physiology

A New Reversed Phase-HPLC Method Resolving All Major Higher Plant Photosynthetic Pigments 1

Javier de las Rivas2, Anunciación Abadía and Javier Abadía

Department of Plant Nutrition, Aula Dei Experimental Station (C.S.I.C.), Apdo. 202, 50080 Zaragoza, Spain

A new reversed phase-high performance liquid chromatography method has been developed to analyze the full complement of higher plant photosynthetic pigments (cis-neoxanthin, neoxanthin, violaxanthin, taraxanthin, anteraxanthin, lutein, zeaxanthin, cis-lutein, chlorophyll b, chlorophyll a, {alpha}- and {beta}-carotene). The separation is carried out on a C18 column in about 10 minutes, using a single high-pressure pump and three different mobile phases in three isocratic steps. This method introduces a major improvement in higher plant photosynthetic pigment analysis, resolving in only 10 minutes all photosynthetic pigments while achieving good separation of lutein from its isomer zeaxanthin. Zeaxanthin is involved in the xanthophyll cycle, which recently has been proposed to play a significant role in the protection of the photosynthetic apparatus from photoinhibitory conditions (Demmig et al. [1987] Plant Physiol 84: 218-224).

2 Recipient of a fellowship from the Servicio de Formación de Personal Investigador, Spanish Ministry of Science and Education. Permanent address: Department of Biochemistry, University of the Basque Country, PO Box 644, 48080 Bilbao, Spain.

1 Supported by grants Dirección General de Investigación Científica y Técnica PB88-0084 and Consejo Asesor de Investigación-Diputacíon General de Aragon CA 8/88 to J. A.




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Copyright © 1989 by the American Society of Plant Biologists