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Microbe-Plant Interactions

Role of the Plasmalemma H⁺-ATPase in Pseudomonas syringae-Induced K⁺/H⁺ Exchange in Suspension-Cultured Tobacco Cells ¹

Microbiology and Plant Pathology Laboratory, Agricultural Research Service, U.S. Department of Agriculture, Beltsville, Maryland 20705

Activation of a host plasma membrane K⁺ efflux/net H⁺ uptake exchange by pathogenic pseudomonads plays an important role in the development of hypersensitivity in tobacco (Nicotiana tabacum). Involvement of the plasmalemma H⁺-pumping ATPase in this response was investigated. The exchange response of suspension-cultured tobacco cells to Pseudomonas syringae pv syringae was reduced 90% or more by ATPase inhibitors including vanadate, N-ethylmaleimide, and N,N'-dicyclohexylcarbodiimide. The exchange was also strongly inhibited by the protonophore carbonyl cyanide m-chlorophenylhydrazone and by slightly alkaline external pH. Respiratory inhibitors such as oligomycin and sodium azide reduced the exchange by 50% to 75%, while glycolysis inhibitors such as sodium arsenite and sodium iodoacetate decreased exchange by approximately 90%. These results suggest that plasmalemma H⁺-ATPase activity is required for the exchange response and that this may reflect a requirement for a plasmalemma pH and/or electrical potential gradient.

¹ Supported in part by U.S. Department of Agriculture Competitive Research Grants Office grants 85-CRCR-1-1779 and 87-CRCR-1-2304.

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