Plant Physiology 91:373-378 (1989)
© 1989 American Society of Plant Biologists
Metabolism and Enzymology
Incorporation of UDP-[14C]Glucose into Xyloglucan by Pea Membranes 1
Ruth Gordon and
Gordon Maclachlan
McGill University, Biology Department, Montreal, Quebec, Canada H3A 1B1
The water-insoluble 1,4- -linked products formed from UDP-[14C]glucose by pea membranes were dissolved in hot dimethyl-sulfoxide/paraformaldehyde and fractionated on columns of controlled pore glass beads calibrated with dextran standards. The products eluted with a peak size close to 70 kilodaltons in dextran equivalents. Similar elution profiles were obtained for products formed in brief or extended incubations and at high or low substrate concentrations. Methylation analysis indicated that only a few [14C]glucose units had been added to an endogenous acceptor to form this product. In the presence of UDP-xylose at concentrations equal to or less than UDP-[14C]glucose, incorporation from the latter was enhanced and the products elongated with time to a size range where the major components eluted between dextran 264 and 500 kilodaltons. Treatment with endo-1,4--glucanase resulted in a mixture of oligosaccharides, including the xyloglucan subunit Glc4Xyl3, which were hydrolyzed further by mixed glycosidases to labeled glucose and isoprimeverose (xylosyl-1,6- -D-glucose). In pulse-chase experiments, the low molecular weight product formed from UDP-[14C]glucose alone was clearly a precursor for high molecular weight products formed subsequently in the presence of both UDP-glucose and UDP-xylose. It is concluded that the 1,4--transglucosylation activity detected in these tests was due to an enzyme that is required for biosynthesis of the backbone of xyloglucan.
1 Supported by grants from the Natural Sciences and Engineering Research Council of Canada.
This article has been cited by other articles:
|
|
|
|
 
F. M. Dwivany, D. Yulia, R. A. Burton, N. J. Shirley, S. M. Wilson, G. B. Fincher, A. Bacic, E. Newbigin, and M. S. Doblin
The CELLULOSE-SYNTHASE LIKE C (CSLC) Family of Barley Includes Members that Are Integral Membrane Proteins Targeted to the Plasma Membrane
Mol Plant,
September 1, 2009;
2(5):
1025 - 1039.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. M. Cavalier, O. Lerouxel, L. Neumetzler, K. Yamauchi, A. Reinecke, G. Freshour, O. A. Zabotina, M. G. Hahn, I. Burgert, M. Pauly, et al.
Disrupting Two Arabidopsis thaliana Xylosyltransferase Genes Results in Plants Deficient in Xyloglucan, a Major Primary Cell Wall Component
PLANT CELL,
June 1, 2008;
20(6):
1519 - 1537.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. M. Cavalier and K. Keegstra
Two Xyloglucan Xylosyltransferases Catalyze the Addition of Multiple Xylosyl Residues to Cellohexaose
J. Biol. Chem.,
November 10, 2006;
281(45):
34197 - 34207.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. R. Urbanowicz, C. Rayon, and N. C. Carpita
Topology of the Maize Mixed Linkage (1->3),(1->4)-{beta}-D-Glucan Synthase at the Golgi Membrane
Plant Physiology,
February 1, 2004;
134(2):
758 - 768.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Faik, N. J. Price, N. V. Raikhel, and K. Keegstra
An Arabidopsis gene encoding an alpha -xylosyltransferase involved in xyloglucan biosynthesis
PNAS,
May 28, 2002;
99(11):
7797 - 7802.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Sarria, T. A. Wagner, M. A. O'Neill, A. Faik, C. G. Wilkerson, K. Keegstra, and N. V. Raikhel
Characterization of a Family of Arabidopsis Genes Related to Xyloglucan Fucosyltransferase1
Plant Physiology,
December 1, 2001;
127(4):
1595 - 1606.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. A. Brummell, C. Catala, C. C. Lashbrook, and A. B. Bennett
A membrane-anchored E-type endo-1,4-beta -glucanase is localized on Golgi and plasma membranes of higher plants
PNAS,
April 29, 1997;
94(9):
4794 - 4799.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
