This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via CrossRef Citing Articles via Google Scholar Google Scholar Articles by Baldi, B. G. Articles by Cohen, J. D. Search for Related Content PubMed PubMed Citation Articles by Baldi, B. G. Articles by Cohen, J. D. Agricola Articles by Baldi, B. G. Articles by Cohen, J. D.

Development and Growth Regulation

Hydrolysis of Indole-3-Acetic Acid Esters Exposed to Mild Alkaline Conditions ¹

U.S. Department of Agriculture-Agricultural Research Service, Plant Hormone Laboratory, Beltsville Agricultural Research Center, Beltsville, Maryland 20705, Department of Botany, University of Maryland, College Park, Maryland 20742

Ester conjugates of indole-3-acetic acid are hydrolyzed easily in basic solutions; however, quantitative data have not been available on the relationship between pH and rate of hydrolysis of the known ester conjugates. The use of basic conditions during extraction or purification of IAA by several laboratories suggested that a more systematic analysis of this process was needed. In this report we present data indicating: (a) that measurable hydrolysis of IAA-glucose (from standard solutions) and IAA-esters (from maize kernel extracts) occurs with only a few hours of treatment at pH 9 or above; (b) that the lability of some ester conjugates is even greater than that of IAA-glucose; and (c) that ester hydrolysis of standard compounds, IAA-glucose and IAA-p-nitrophenol, occurs in the `three phase extraction system' proposed by Liu and Tillberg ([1983] Physiol Plant 57: 441-447). These data indicate that the potential for problems with inadvertent hydrolysis of ester conjugates of IAA exists even at moderate pH values and in the multiphase system where exposure to basic conditions was thought to be limited.

This article has been cited by other articles:

				A. W. WOODWARD and B. BARTEL Auxin: Regulation, Action, and Interaction Ann. Bot., April 1, 2005; 95(5): 707 - 735. [Abstract] [Full Text] [PDF]

				K. N'K. Mwange, H.-W. Hou, and K.-M. Cui Relationship between endogenous indole-3-acetic acid and abscisic acid changes and bark recovery in Eucommia ulmoides Oliv. after girdling J. Exp. Bot., August 1, 2003; 54(389): 1899 - 1907. [Abstract] [Full Text] [PDF]

				M. Kowalczyk and G. Sandberg Quantitative Analysis of Indole-3-Acetic Acid Metabolites in Arabidopsis Plant Physiology, December 1, 2001; 127(4): 1845 - 1853. [Abstract] [Full Text] [PDF]